The MF-Human IL-6 (Interleukin 6) ELISA Kit: An Advanced Tool for Cytokine Quantification
The MF-Human IL-6 (Interleukin 6) ELISA Kit is a highly specialized and essential tool for the precise quantification of IL-6 levels in various human biological samples, including serum, plasma, and cell culture supernatants. IL-6 is a multifunctional cytokine that plays a crucial role in the immune response, inflammation, and hematopoiesis. Accurate measurement of IL-6 is vital in understanding its role in pathophysiological conditions such as inflammatory diseases, autoimmune disorders, infections, and cancers.
Key Features and Benefits
1.Quantitative Measurement:
Provides precise and accurate quantification of IL-6 levels, critical for both research and clinical diagnostics.
2.High Sensitivity and Specificity:
The assay is designed to detect IL-6 with high sensitivity, allowing for the detection of low concentrations.
Exhibits minimal cross-reactivity with other cytokines, ensuring specific measurement of IL-6.
3.User-Friendly Protocol:
The kit includes all necessary reagents and detailed instructions, making it accessible for both novice and experienced users.
4.Wide Sample Compatibility:
Suitable for a range of human samples, including serum, plasma, and cell culture supernatants.
5.Rapid and Reliable Results:
The entire assay can be completed within a few hours, providing timely results for urgent analyses.
Components of the Kit
Pre-coated 96-well ELISA Plate:
Wells pre-coated with monoclonal antibodies specific to human IL-6.
IL-6 Standards and Controls:
Pre-diluted IL-6 standards for generating a standard curve and quality controls to ensure assay accuracy and reliability.
Detection Reagents:
Includes biotinylated anti-IL-6 detection antibodies and streptavidin-HRP (Horseradish Peroxidase) conjugate.
Substrate Solution:
TMB (3,3',5,5'-Tetramethylbenzidine) substrate for color development.
Stop Solution:
Typically a sulfuric or phosphoric acid solution to halt the enzymatic reaction.
Wash Buffer and Dilution Buffer:
Buffers for washing the plates and diluting samples or reagents as needed.
Detailed Assay Procedure
1.Sample Preparation:
Collect human biological samples (serum, plasma, or cell culture supernatants) and filter or centrifuge to remove any particulate matter.
2.Plate Preparation:
Add IL-6 standards, controls, and prepared samples to the pre-coated wells.
3.Incubation:
Incubate the plate to allow IL-6 in the samples to bind to the immobilized antibodies on the plate.
4.Detection:
Add biotinylated anti-IL-6 detection antibody followed by streptavidin-HRP, and incubate to form the antibody-antigen complex.
5.Substrate Addition:
Add TMB substrate to the wells and incubate to develop a colorimetric signal proportional to the amount of IL-6 in the samples.
6.Reaction Termination:
Add stop solution to halt the reaction, changing the color from blue to yellow for better absorbance measurement.
7.Measurement:
Measure the absorbance at 450 nm using a microplate reader. The intensity of the color is directly proportional to the IL-6 concentration.
Applications in Research and Clinical Diagnostics
1.Inflammatory and Autoimmune Diseases:
Measurement of IL-6 is crucial in studying inflammatory diseases like rheumatoid arthritis, lupus, and inflammatory bowel disease.
2.Cancer Research:
IL-6 levels are often elevated in various cancers, including lymphoma, breast cancer, and prostate cancer. Monitoring IL-6 can aid in understanding tumor progression and treatment efficacy.
3.Infectious Diseases:
IL-6 is a key marker in the body’s response to infections, including viral, bacterial, and fungal infections.
4.Therapeutic Monitoring:
Used to monitor patients undergoing therapies that target IL-6 pathways, such as monoclonal antibodies against IL-6 or its receptor.
5.Basic Research:
Facilitates studies on cytokine biology, immune regulation, and the molecular mechanisms underlying various diseases.
Conclusion
The MF-Human IL-6 (Interleukin 6) ELISA Kit is an invaluable tool for researchers and clinicians, providing a reliable, sensitive, and specific method for measuring IL-6 in human samples. By offering detailed and accurate insights into IL-6 levels, this kit supports a wide range of applications from basic research to clinical diagnostics and therapeutic monitoring, ultimately contributing to the advancement of knowledge and treatment in various medical fields.
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